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Luminex xMAP - Technology
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Principle of the Luminex - Technology:
- Polystyrene microspheres internally dyed with spectrally distinct fluorochromes define a set of signature beads (Bead Mix).
- Each set of signature beads carries a specific detection reagent (such as oligonucleotide probes, recombinant antigens or specific proteins) on its surface
- Each set of signature beads carries a specific detection reagent (such as oligonucleotide probes, recombinant antigens or specific proteins) on its surface.
- Analytes contained in added samples bind to detection reagents on beads.
- A reporter coupled with another fluorochrome quantifies the reaction on the bead surface.
- The biomolecular interactions are quantified by the Luminex LX 100 IS / 200 analyzer while passing the laser beams.
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Advantages of the Luminex technology:
- Small sample volume
- High sensitivity
- High specificity
- Fully automated readout of results
- Linearity of signal intensity for up to 4 orders of magnitude
- Statistical analysis of each single analyte leads to unexcelled reliability
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For more information on available assays, please see our Luminex product list or contact us.
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